Extracellular Blockade by TEA
Molecular dynamics and umbrella sampling calculations are used to explore extracellular blockade of the
KcsA by tetraethylamonium (TEA), a classical blocker of K+ channels. It is found, in remarkable agreement
with experiment, that TEA is more stably bound when there are aromatic residues at position 82.
The enhanced stability conferred by the aromatic residues does not arise from
Pi-cation interactions as was previously suggested, but is due to hydrophobic interactions.
S. CROUZY, S. BERNÈCHE and B. ROUX, "Extracellular Blockade of K+ Channels by TEA:
Results from Molecular Dynamics Simulations of the KcsA channel",
J. Gen. Physiol. 118, 207-216 (2001).